I've been screwing up the same experiment for nearly two weeks now. I tried everything I could think of to fix it. "It" is a western (an experiment to look for presence/ absence of protein and a rough estimate of levels of protein). You have to make a gel that you put your protein into and then run electricity through the gel causing the protein to move down the gel. Depending on the size of your particular protein, it "run"down the gel at different speeds. Bigger= slower, smaller=faster. Anyways, my problem is that mine keeps running funny. It makes a frowny face and the first lane that has several colors in it scrunches up on the top and then spreads too far out about half way down. I had a new idea on how to fix it so I'm going to try again in about ten minutes. I'll let you know how it goes.
--update--
Catarina just ran a gel independent of all of my reagents and the same thing happened to her. I might hate science today.
--update the second--
It looked fine which makes me annoyed. I guess I can go ahead and do the real experiment. Hopefully my luck will last that long.
No comments:
Post a Comment